Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0896Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 40ng/ml | 1.877 | 1.915 | 1.869 |
| 1.953 | |||
| 20ng/ml | 1.085 | 1.095 | 1.049 |
| 1.104 | |||
| 10ng/ml | 0.421 | 0.435 | 0.389 |
| 0.449 | |||
| 5ng/ml | 0.265 | 0.222 | 0.176 |
| 0.179 | |||
| 2.5ng/ml | 0.068 | 0.06 | 0.015 |
| 0.053 | |||
| 0ng/ml | 0.034 | 0.046 | 0 |
| 0.057 |
Precision
We measured random samples of E0896Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 13.4 | 0.4 | 3.2 |
| Intra-assay | 2 | 18 | 33.7 | 1.5 | 4.5 |
| Intra-assay | 3 | 18 | 21.2 | 1 | 4.5 |
Protocols
References
Customer reviews and Q&As
