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Home > Product > ELISA Kit > Human Phospholipase C, PLC ELISA Kit

Human Phospholipase C, PLC ELISA Kit

Human Phospholipase C, PLC ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.096ng/mL

Detection range: 0.2-60ng/mL

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.096ng/mL

Detection range: 0.2-60ng/mL

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product overview

Full product name

Phospholipase C

Code

E0788Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.096ng/mL

Detection range

0.2-60ng/mL

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species

Human

Storage

2-8°C

Assay time

1h 30m

Background

Phospholipase C releases diacylglycerol by cleaving the glycosylphosphatidylinositol. It has broad substrate specificity and its activity is influenced by metal ions and surfactants.

Research area

Signal Transduction|Cardiovascular|Metabolism

Target protein

PLC

Components

Components Quantity
Standard Solution 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Antibody 1ml x1

Protocol data

These standard curves of E0788Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/mL 1.804 1.91 1.878
2.016
16ng/mL 1.095 1.052 1.02
1.008
8ng/mL 0.473 0.462 0.43
0.451
4ng/mL 0.166 0.213 0.181
0.26
2ng/mL 0.055 0.076 0.045
0.098
0ng/mL 0.034 0.032 0
0.03

Precision

We measured random samples of E0788Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 13.1 0.6 4.3
Intra-assay 2 18 24.5 0.7 2.8
Intra-assay 3 18 8.4 0.3 3.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0788Hu

    $320.00

  • 96T

    E0788Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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