Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0289Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 320pg/ml | 2.188 | 2.274 | 2.243 |
| 2.359 | |||
| 160pg/ml | 1.395 | 1.333 | 1.301 |
| 1.271 | |||
| 80pg/ml | 0.744 | 0.753 | 0.722 |
| 0.762 | |||
| 40pg/ml | 0.432 | 0.425 | 0.393 |
| 0.418 | |||
| 20pg/ml | 0.141 | 0.143 | 0.112 |
| 0.146 | |||
| 0pg/ml | 0.03 | 0.031 | 0 |
| 0.032 |
Precision
We measured random samples of E0289Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 200.1 | 5.3 | 2.6 |
| Intra-assay | 2 | 18 | 53.3 | 3.1 | 5.7 |
| Intra-assay | 3 | 18 | 111.3 | 5.7 | 5.1 |
Protocols
References
Customer reviews and Q&As
