Product overview
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Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1593Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/ml | 1.962 | 1.895 | 1.843 |
| 1.828 | |||
| 12ng/ml | 0.908 | 0.943 | 0.891 |
| 0.977 | |||
| 6ng/ml | 0.434 | 0.482 | 0.43 |
| 0.531 | |||
| 3ng/ml | 0.296 | 0.22 | 0.168 |
| 0.143 | |||
| 1.5ng/ml | 0.087 | 0.099 | 0.047 |
| 0.111 | |||
| 0ng/ml | 0.059 | 0.052 | 0 |
| 0.044 |
Precision
We measured random samples of E1593Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 13 | 0.6 | 4.7 |
| Intra-assay | 2 | 18 | 16.6 | 0.5 | 2.8 |
| Intra-assay | 3 | 18 | 4.8 | 0.2 | 4.3 |
Protocols
References
Customer reviews and Q&As
