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Home > Product > ELISA Kit > Mouse Heparan Sulfate Glucosamine 3-O-sulfotransferase 1, HS3ST1 ELISA Kit

Mouse Heparan Sulfate Glucosamine 3-O-sulfotransferase 1, HS3ST1 ELISA Kit

Mouse Heparan Sulfate Glucosamine 3-O-sulfotransferase 1, HS3ST1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.16ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.16ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Heparan Sulfate Glucosamine 3-O-Sulfotransferase 1

Code

E2126Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.16ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.

UniProt accession

MASS(Da)

35,899

GeneID

15476

Synonyms

Heparan sulfate 3-O-sulfotransferase 1;Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1;Heparan sulfate glucosamine 3-O-sulfotransferase 1;HS3ST1

Gene names

HS3ST1

Research area

Immunology

Target protein

HS3ST1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2126Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.323 2.246 2.205
2.169
1200ng/L 1.649 1.699 1.658
1.749
600ng/L 1.126 1.076 1.036
1.027
300ng/L 0.723 0.681 0.641
0.64
150ng/L 0.452 0.464 0.423
0.476
0ng/L 0.045 0.041 0
0.036

Precision

We measured random samples of E2126Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 360.4 21.18 5.9
Intra-Assay 2 18 867.1 39 4.5
Intra-Assay 3 18 278.6 19.85 7.1

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E2126Mo

    $320.00

  • 96T

    E2126Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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