Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E4269Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 8ng/ml | 1.732 | 1.858 | 1.804 |
| 1.983 | |||
| 4ng/ml | 1.005 | 0.939 | 0.886 |
| 0.873 | |||
| 2ng/ml | 0.505 | 0.567 | 0.513 |
| 0.628 | |||
| 1ng/ml | 0.208 | 0.23 | 0.177 |
| 0.252 | |||
| 0.5ng/ml | 0.115 | 0.11 | 0.056 |
| 0.104 | |||
| 0ng/ml | 0.054 | 0.053 | 0 |
| 0.052 |
Precision
We measured random samples of E4269Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 3 | 0.1 | 3.5 |
| Intra-assay | 2 | 18 | 6 | 0.2 | 2.7 |
| Intra-assay | 3 | 18 | 3.5 | 0.2 | 4.9 |
Protocols
References
Customer reviews and Q&As
