Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0072Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 4ng/ml | 2.039 | 2.062 | 1.995 |
| 2.086 | |||
| 2ng/ml | 1.075 | 1.107 | 1.039 |
| 1.139 | |||
| 1ng/ml | 0.593 | 0.605 | 0.538 |
| 0.618 | |||
| 0.5ng/ml | 0.294 | 0.302 | 0.234 |
| 0.309 | |||
| 0.25ng/ml | 0.172 | 0.182 | 0.114 |
| 0.191 | |||
| 0ng/ml | 0.071 | 0.068 | 0 |
| 0.064 |
Precision
We measured random samples of E0072Bo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 1.9 | 0.1 | 4.4 |
| Intra-assay | 2 | 18 | 2.8 | 0.1 | 2.1 |
| Intra-assay | 3 | 18 | 1.1 | 0 | 4.4 |
Protocols
References
Customer reviews and Q&As
