Product overview
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Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0785Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 64umol/L | 2.115 | 2.083 | 2 |
| 2.051 | |||
| 32umol/L | 1.175 | 1.142 | 1.06 |
| 1.11 | |||
| 16umol/L | 0.581 | 0.602 | 0.519 |
| 0.623 | |||
| 8umol/L | 0.308 | 0.32 | 0.237 |
| 0.332 | |||
| 4umol/L | 0.179 | 0.185 | 0.103 |
| 0.192 | |||
| 0umol/L | 0.081 | 0.083 | 0 |
| 0.085 |
Precision
We measured random samples of E0785Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 36.2 | 1.7 | 4.7 |
| Intra-assay | 2 | 18 | 50.2 | 1.6 | 3.1 |
| Intra-assay | 3 | 18 | 10.9 | 0.6 | 5.2 |
Protocols
References
Customer reviews and Q&As
