Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E3642Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 16nmol/ml | 2.2 | 2.186 | 2.134 |
| 2.173 | |||
| 8nmol/ml | 1.336 | 1.443 | 1.391 |
| 1.55 | |||
| 4nmol/ml | 0.841 | 0.836 | 0.784 |
| 0.832 | |||
| 2nmol/ml | 0.467 | 0.471 | 0.418 |
| 0.474 | |||
| 1nmol/ml | 0.298 | 0.258 | 0.206 |
| 0.218 | |||
| 0nmol/ml | 0.064 | 0.052 | 0 |
| 0.041 |
Precision
We measured random samples of E3642Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 11.3 | 0.5 | 4.1 |
| Intra-assay | 2 | 18 | 6.3 | 0.4 | 6.4 |
| Intra-assay | 3 | 18 | 3.2 | 0.2 | 5.8 |
Protocols
References
Customer reviews and Q&As
