Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1414Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 4000ng/L | 2.148 | 2.06 | 2.024 |
| 1.972 | |||
| 2000ng/L | 1.169 | 1.079 | 1.043 |
| 0.988 | |||
| 1000ng/L | 0.565 | 0.574 | 0.538 |
| 0.582 | |||
| 500ng/L | 0.266 | 0.273 | 0.237 |
| 0.28 | |||
| 250ng/L | 0.121 | 0.122 | 0.085 |
| 0.122 | |||
| 0ng/L | 0.046 | 0.036 | 0 |
| 0.026 |
Precision
We measured random samples of E1414Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 2683 | 69.9 | 2.6 |
| Intra-assay | 2 | 18 | 1321.9 | 70.9 | 5.4 |
| Intra-assay | 3 | 18 | 1695.3 | 66.3 | 3.9 |
Protocols
References
Customer reviews and Q&As
