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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0025Ch are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.036	2.09	2.024
	2.144
320ng/L	1.192	1.168	1.101
	1.144
160ng/L	0.569	0.582	0.516
	0.595
80ng/L	0.326	0.337	0.271
	0.349
40ng/L	0.171	0.161	0.095
	0.151
0ng/L	0.065	0.067	0
	0.068

We measured random samples of E0025Ch within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	222.5	10.5	4.7
Intra-assay	2	18	447.6	13.9	3.1
Intra-assay	3	18	102	5.1	5

> Protocol Booklet

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