Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0025Ch are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 640ng/L | 2.036 | 2.09 | 2.024 |
| 2.144 | |||
| 320ng/L | 1.192 | 1.168 | 1.101 |
| 1.144 | |||
| 160ng/L | 0.569 | 0.582 | 0.516 |
| 0.595 | |||
| 80ng/L | 0.326 | 0.337 | 0.271 |
| 0.349 | |||
| 40ng/L | 0.171 | 0.161 | 0.095 |
| 0.151 | |||
| 0ng/L | 0.065 | 0.067 | 0 |
| 0.068 |
Precision
We measured random samples of E0025Ch within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 222.5 | 10.5 | 4.7 |
| Intra-assay | 2 | 18 | 447.6 | 13.9 | 3.1 |
| Intra-assay | 3 | 18 | 102 | 5.1 | 5 |
Protocols
References
Customer reviews and Q&As
