Full product name

Size

Sensitivity

Detection range

Sample type

Code

Assay type

Species

Storage

Background

Research area

Target protein

Components	Quantity
Pre-coated Plate	12 * 8 well strips x 1
Standard, lyophilized	2 vials
Standard/Sample Diluent	6ml ×1 vial
Biotinylated Antigen, lyophilized	1 vial
Avidin-HRP Concentrate	100μl × 1 vial
Biotinylated Antigen Diluent	6ml × 1 vials
Avidin HRP Diluent	5.9ml × 1 vials
Substrate Solution A	6ml ×1 vial
Substrate Solution B	6ml ×1 vial
Stop Solution	6ml ×1 vial
Wash Buffer Concentrate (25x)	20ml × 1 vial

These standard curves of EA0141Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
0ng/ml	2.059	2.046	2.003
	2.034
0.4ng/ml	1.738	1.766	1.723
	1.794
0.8ng/ml	1.283	1.224	1.181
	1.165
1.6ng/ml	0.743	0.717	0.674
	0.69
3.2ng/ml	0.387	0.411	0.368
	0.435
6.4ng/ml	0.199	0.183	0.14
	0.166

We measured random samples of EA0141Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	2	0.1	5.5
Intra-assay	2	18	0.7	0	3.9
Intra-assay	3	18	1.1	0.1	5.5

> Protocol Booklet

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