Product overview
Full product name
Size
Sensitivity
Detection range
Sample type
Code
Assay type
Species
Storage
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated Plate | 12 * 8 well strips x 1 |
| Standard, lyophilized | 2 vials |
| Standard/Sample Diluent | 6ml ×1 vial |
| Biotinylated Antigen, lyophilized | 1 vial |
| Avidin-HRP Concentrate | 100μl × 1 vial |
| Biotinylated Antigen Diluent | 6ml × 1 vials |
| Avidin HRP Diluent | 5.9ml × 1 vials |
| Substrate Solution A | 6ml ×1 vial |
| Substrate Solution B | 6ml ×1 vial |
| Stop Solution | 6ml ×1 vial |
| Wash Buffer Concentrate (25x) | 20ml × 1 vial |
Protocol data
These standard curves of EA0141Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 0ng/ml | 2.059 | 2.046 | 2.003 |
| 2.034 | |||
| 0.4ng/ml | 1.738 | 1.766 | 1.723 |
| 1.794 | |||
| 0.8ng/ml | 1.283 | 1.224 | 1.181 |
| 1.165 | |||
| 1.6ng/ml | 0.743 | 0.717 | 0.674 |
| 0.69 | |||
| 3.2ng/ml | 0.387 | 0.411 | 0.368 |
| 0.435 | |||
| 6.4ng/ml | 0.199 | 0.183 | 0.14 |
| 0.166 |
Precision
We measured random samples of EA0141Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 2 | 0.1 | 5.5 |
| Intra-assay | 2 | 18 | 0.7 | 0 | 3.9 |
| Intra-assay | 3 | 18 | 1.1 | 0.1 | 5.5 |
Protocols
References
Customer reviews and Q&As
