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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0080Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	1.67	1.795	1.758
	1.92
1600ng/L	0.905	0.913	0.876
	0.92
800ng/L	0.548	0.571	0.534
	0.593
400ng/L	0.264	0.233	0.196
	0.201
200ng/L	0.106	0.089	0.052
	0.071
0ng/L	0.037	0.037	0
	0.037

We measured random samples of E0080Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	1152	67.3	5.8
Intra-assay	2	18	1434.1	64.8	4.5
Intra-assay	3	18	2572.2	66.1	2.6

> Protocol Booklet

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