Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0080Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 3200ng/L | 1.67 | 1.795 | 1.758 |
| 1.92 | |||
| 1600ng/L | 0.905 | 0.913 | 0.876 |
| 0.92 | |||
| 800ng/L | 0.548 | 0.571 | 0.534 |
| 0.593 | |||
| 400ng/L | 0.264 | 0.233 | 0.196 |
| 0.201 | |||
| 200ng/L | 0.106 | 0.089 | 0.052 |
| 0.071 | |||
| 0ng/L | 0.037 | 0.037 | 0 |
| 0.037 |
Precision
We measured random samples of E0080Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 1152 | 67.3 | 5.8 |
| Intra-assay | 2 | 18 | 1434.1 | 64.8 | 4.5 |
| Intra-assay | 3 | 18 | 2572.2 | 66.1 | 2.6 |
Protocols
References
Customer reviews and Q&As
