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Home > Product > ELISA Kit > Mouse Interleukin-36 Beta, IL1F8 ELISA Kit

Mouse Interleukin-36 Beta, IL1F8 ELISA Kit

Mouse Interleukin-36 Beta, IL1F8 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.47ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 1.47ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Interleukin-36 Beta

Code

E2192Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.47ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial Peptides including beta-defensin 4 and beta-defensin 103 as well as a number of matrix metalloproteases (By similarity). Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. Induces the production of proinflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23, and activates p38 MAPK phosphorylation in BMDCs. Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by T-helper 1 (Th1) cells, cultured CD4(+) T-cells and splenocytes.

UniProt accession

MASS(Da)

20,878

GeneID

69677

Synonyms

IL 36B;IL-1F8;IL36B;Interleukin-1 family member 8;Interleukin-36 beta

Gene names

IL36B

Research area

Immunology

Target protein

IL36B

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2192Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 2.508 2.479 2.434
2.449
240ng/L 1.428 1.411 1.366
1.393
120ng/L 0.859 0.836 0.791
0.812
60ng/L 0.526 0.485 0.44
0.444
30ng/L 0.206 0.22 0.175
0.233
0ng/L 0.061 0.045 0
0.029

Precision

We measured random samples of E2192Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 104 6.79 6.5
Intra-Assay 2 18 288 10.65 3.7
Intra-Assay 3 18 107.8 6.41 5.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E2192Mo

    $320.00

  • 96T

    E2192Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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