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Home > Product > ELISA Kit > Mouse Lymphocyte Activation Gene 3 Protein, LAG3 ELISA Kit

Mouse Lymphocyte Activation Gene 3 Protein, LAG3 ELISA Kit

Mouse Lymphocyte Activation Gene 3 Protein, LAG3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.261ng/mL

Detection range: 0.5-150ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.261ng/mL

Detection range: 0.5-150ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Lymphocyte Activation Gene 3 Protein

Code

E2220Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.261ng/mL

Detection range

0.5-150ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells (PubMed: 12209638, PubMed: 12421911, PubMed: 12672063, PubMed: 15100286, PubMed: 15634887, PubMed: 30580966). Delivers inhibitory signals upon binding to ligands, such as FGL1 (PubMed: 30580966). FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (PubMed: 30580966). Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed: 12209638, PubMed: 12421911, PubMed: 12672063, PubMed: 15100286, PubMed: 15634887). May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (PubMed: 21300912). Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells (PubMed: 12209638, PubMed: 12421911, PubMed: 12672063, PubMed: 15100286, PubMed: 15634887). Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (PubMed: 15485628). Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (PubMed: 19201841). Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear (PubMed: 12209638, PubMed: 12421911, PubMed: 15634887). [Secreted lymphocyte activation gene 3 protein]: May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response.

UniProt accession

MASS(Da)

56,978

GeneID

16768

Synonyms

Activation-induced cytidine deaminase-linked autoimmunity protein;Aida;CD antigen CD223;LAG 3;LAG3;LAG-3;Lymphocyte activation gene 3 protein;Secreted lymphocyte activation gene 3 protein;sLAG-3;

Gene names

LAG3

Research area

Immunology

Target protein

LAG3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2220Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
80ng/mL 2.085 2.139 2.093
2.194
40ng/mL 1.558 1.536 1.489
1.514
20ng/mL 0.736 0.837 0.79
0.938
10ng/mL 0.405 0.41 0.363
0.415
5ng/mL 0.272 0.254 0.207
0.236
0ng/mL 0.049 0.047 0
0.045

Precision

We measured random samples of E2220Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 46 1.93 4.2
Intra-Assay 2 18 15.2 0.77 5.1
Intra-Assay 3 18 26.5 1.3 4.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E2220Mo

    $320.00

  • 96T

    E2220Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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