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Home > Product > ELISA Kit > Mouse Nacht, LRR and Pyd Domains-containing Protein 3, NLRP3 ELISA Kit

Mouse Nacht, LRR and Pyd Domains-containing Protein 3, NLRP3 ELISA Kit

Mouse Nacht, LRR and Pyd Domains-containing Protein 3, NLRP3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.66ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 1.66ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Nacht, Lrr and Pyd Domains-Containing Protein 3

Code

E2283Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.66ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (or possibly CASP4/CASP11). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu (PubMed: 28847925, PubMed: 27374331). Activation of NLRP3 inflammasome is also required for HMGB1 secretion (PubMed: 22801494). The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K(+) efflux, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, cytosolic dsRNA, etc. However, it is unclear what constitutes the direct NLRP3 activator. Activation in presence of cytosolic dsRNA is mediated by DHX33 (By similarity). Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth. During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (PubMed: 26098997).

UniProt accession

MASS(Da)

118,275

GeneID

216799

Synonyms

Cold autoinflammatory syndrome 1 protein homolog;Cryopyrin;Mast cell maturation-associated-inducible protein 1;NACHT, LRR and PYD domains-containing protein 3;NLRP 3;NLRP3;PYRIN-containing APAF1-like protein 1

Gene names

NLRP3

Research area

Cell Biology

Target protein

NLRP3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2283Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 1.746 1.756 1.708
1.766
240ng/L 1.141 1.068 1.02
0.996
120ng/L 0.427 0.492 0.444
0.558
60ng/L 0.219 0.249 0.201
0.279
30ng/L 0.058 0.074 0.026
0.09
0ng/L 0.047 0.048 0
0.049

Precision

We measured random samples of E2283Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 143.5 5.58 3.9
Intra-Assay 2 18 288 14.46 5
Intra-Assay 3 18 315.4 11.29 3.6

Protocols

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  • Size

    Code

    Price

  • 48T

    E2283Mo

    $320.00

  • 96T

    E2283Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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