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Home > Product > ELISA Kit > Mouse Phosphatidylcholine-sterol Acyltransferase, LCAT ELISA Kit

Mouse Phosphatidylcholine-sterol Acyltransferase, LCAT ELISA Kit

Mouse Phosphatidylcholine-sterol Acyltransferase, LCAT ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.11ng/mL

Detection range: 0.2-60ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.11ng/mL

Detection range: 0.2-60ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Phosphatidylcholine-Sterol Acyltransferase

Code

E2340Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.11ng/mL

Detection range

0.2-60ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs) (PubMed: 19065001). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels (PubMed: 19065001). Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins (PubMed: 19065001). Required for remodeling high-density lipoprotein particles into their spherical forms (PubMed: 19065001). Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines (PubMed: 8820107). Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (PubMed: 10393212). Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF (By similarity). Catalyzes the esterification of (24S)-hydroxycholesterol (24(S)OH-C), also known as cerebrosterol to produce 24(S)OH-C monoesters (By similarity).

UniProt accession

MASS(Da)

49,747

GeneID

16816

Synonyms

1-alkyl-2-acetylglycerophosphocholine esterase;LCAT;Lecithin-cholesterol acyltransferase;PAF acetylhydrolase;Phosphatidylcholine-sterol acyltransferase;Phospholipid-cholesterol acyltransferase;Platelet-activating factor acetylhydrolase

Gene names

LCAT

Research area

Cardiovascular

Target protein

LCAT

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2340Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/mL 2.221 2.279 2.234
2.338
16ng/mL 1.524 1.53 1.485
1.537
8ng/mL 1.025 1.089 1.044
1.153
4ng/mL 0.756 0.719 0.674
0.683
2ng/mL 0.446 0.459 0.414
0.471
0ng/mL 0.058 0.045 0
0.032

Precision

We measured random samples of E2340Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%

Protocols

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2340Mo

    $320.00

  • 96T

    E2340Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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