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Home > Product > ELISA Kit > Mouse Toll-like Receptor 5, TLR-5 ELISA Kit

Mouse Toll-like Receptor 5, TLR-5 ELISA Kit

Mouse Toll-like Receptor 5, TLR-5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 14.05ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 14.05ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Toll-Like Receptor 5

Code

E2515Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

14.05ng/L

Detection range

30-9000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life.

UniProt accession

MASS(Da)

97,627

GeneID

53791

Synonyms

TLR 5;TLR5;Toll-like receptor 5

Gene names

TLR5

Research area

Immunology

Target protein

TLR5

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2515Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4800ng/L 2.495 2.419 2.383
2.343
2400ng/L 1.535 1.529 1.494
1.523
1200ng/L 0.779 0.848 0.812
0.917
600ng/L 0.491 0.471 0.435
0.45
300ng/L 0.281 0.246 0.21
0.211
0ng/L 0.039 0.035 0
0.032

Precision

We measured random samples of E2515Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2021.6 60.98 3
Intra-Assay 2 18 1048.4 56.68 5.4
Intra-Assay 3 18 746.5 41.43 5.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E2515Mo

    $320.00

  • 96T

    E2515Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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