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Home > Product > ELISA Kit > Mouse Triggering Receptor Expressed On Myeloid Cells 2, TREM2 ELISA Kit

Mouse Triggering Receptor Expressed On Myeloid Cells 2, TREM2 ELISA Kit

Mouse Triggering Receptor Expressed On Myeloid Cells 2, TREM2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Triggering Receptor Expressed on Myeloid Cells 2

Code

E2525Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed: 11241283). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia (PubMed: 27477018, PubMed: 29518356). Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokines, such as IL6R and CCL3, and the anti-inflammatory cytokine ARG1 (PubMed: 27477018, PubMed: 29518356). Acts as a receptor for lipoprotein particles such as LDL, VLDL, and HDL and for apolipoproteins such as APOA1, APOA2, APOB, APOE, APOE2, APOE3, APOE4, and CLU and enhances their uptake in microglia (PubMed: 27477018). Binds phospholipids (preferably anionic lipids) such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin (By similarity). Regulates microglial proliferation by acting as an upstream regulator of the Wnt/beta-catenin signaling cascade (PubMed: 28077724). Required for microglial phagocytosis of apoptotic neurons (PubMed: 24990881). Also required for microglial activation and phagocytosis of myelin debris after neuronal injury and of neuronal synapses during synapse elimination in the developing brain (PubMed: 15728241, PubMed: 28592261, PubMed: 29752066, PubMed: 25631124). Regulates microglial chemotaxis and process outgrowth, and also the microglial response to oxidative stress and lipopolysaccharide (PubMed: 30232263, PubMed: 29663649, PubMed: 28483841, PubMed: 29859094). It suppresses PI3K and NF-kappa-B signaling in response to lipopolysaccharide; thus promoting phagocytosis, suppressing pro-inflammatory cytokine and nitric oxide production, inhibiting apoptosis and increasing expression of IL10 and TGFB (PubMed: 29663649). During oxidative stress, it promotes anti-apoptotic NF-kappa-B signaling and ERK signaling (PubMed: 28592261). Plays a role in microglial MTOR activation and metabolism (PubMed: 28802038). Regulates age-related changes in microglial numbers (PubMed: 30548312, PubMed: 29752066, PubMed: 25631124). Triggers activation of the immune responses in macrophages and dendritic cells (By similarity). Mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (PubMed: 18957693). In dendritic cells, it mediates up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (By similarity). Involved in the positive regulation of osteoclast differentiation (PubMed: 16418779).

UniProt accession

MASS(Da)

24,527

GeneID

83433

Synonyms

TREM 2;TREM2;TREM-2;Triggering receptor expressed on monocytes 2;Triggering receptor expressed on myeloid cells 2

Gene names

TREM2

Research area

Immunology

Target protein

TREM2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2525Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 2.333 2.249 2.21
2.165
6ng/mL 1.353 1.374 1.335
1.395
3ng/mL 0.649 0.677 0.638
0.705
1.5ng/mL 0.441 0.484 0.446
0.528
0.75ng/mL 0.241 0.207 0.169
0.173
0ng/mL 0.052 0.038 0
0.025

Precision

We measured random samples of E2525Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.1 0.13 3.3
Intra-Assay 2 18 5.7 0.18 3.2
Intra-Assay 3 18 2.2 0.14 6.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E2525Mo

    $320.00

  • 96T

    E2525Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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