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Home > Product > ELISA Kit > Mouse Vascular Endothelial Cell Growth Factor Receptor 2, VEGFR-2 ELISA Kit

Mouse Vascular Endothelial Cell Growth Factor Receptor 2, VEGFR-2 ELISA Kit

Mouse Vascular Endothelial Cell Growth Factor Receptor 2, VEGFR-2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.55ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 1.55ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Vascular Endothelial Cell Growth Factor Receptor 2

Code

E2557Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.55ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as a negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and by preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.

UniProt accession

MASS(Da)

150,462

GeneID

16542

Synonyms

CD antigen CD309;Fetal liver kinase 1;FLK-1;KDR;Kinase NYK;Protein-tyrosine kinase receptor flk-1;Vascular endothelial growth factor receptor 2;VEGFR-2

Gene names

KDR

Research area

Cancer

Target protein

KDR

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2557Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 1.67 1.76 1.715
1.85
240ng/L 1.094 1.084 1.039
1.073
120ng/L 0.609 0.608 0.563
0.607
60ng/L 0.145 0.204 0.159
0.264
30ng/L 0.15 0.114 0.069
0.078
0ng/L 0.046 0.045 0
0.044

Precision

We measured random samples of E2557Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 242.3 11.07 4.6
Intra-Assay 2 18 393.8 20.64 5.2
Intra-Assay 3 18 103 4.56 4.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E2557Mo

    $320.00

  • 96T

    E2557Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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