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Home > Product > ELISA Kit > Porcine Clusterin, CLU ELISA Kit

Porcine Clusterin, CLU ELISA Kit

Porcine Clusterin, CLU ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.59ng/mL

Detection range: 1-400ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 0.59ng/mL

Detection range: 1-400ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Clusterin

Code

E0545Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.59ng/mL

Detection range

1-400ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Functions as extracellular chaperone that prevents aggregation of non native proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity (By similarity). Following stress, promotes apoptosis (By similarity). Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation. An intracellular form suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5. Secreted form does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity (By similarity). Secreted form act as an important modulator during neuronal differentiation through interaction with STMN3 (By similarity). Plays a role in the clearance of immune complexes that arise during cell injury (By similarity).

UniProt accession

MASS(Da)

51,775

GeneID

397025

Synonyms

CLI;CLU;Clusterin;Clusterin alpha chain;Clusterin beta chain;Complement cytolysis inhibitor;CP40

Gene names

CLU

Research area

Immunology

Target protein

CLU

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0545Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240ng/mL 2.327 2.323 2.256
2.319
120ng/mL 1.381 1.427 1.36
1.472
60ng/mL 0.732 0.782 0.715
0.832
30ng/mL 0.448 0.486 0.419
0.525
15ng/mL 0.301 0.307 0.24
0.313
0ng/mL 0.076 0.067 0
0.058

Precision

We measured random samples of E0545Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 165.2 5.15 3.1
Intra-Assay 2 18 21.9 1.27 5.8
Intra-Assay 3 18 62.8 3.61 5.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E0545Po

    $320.00

  • 96T

    E0545Po

    $458.00

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Lead time: Within two weeks

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