Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Humanin (HN) is the first member of a new class of small signaling peptides that originate from the mitochondrial genome. First discovered as a neuroprotective molecule that protected neurons against amyloid-beta toxicity, it has additionally been shown to have anti-apoptotic and cytoprotective properties.
UniProt accession
MASS（Da）
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E4823Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 960ng/L | 2.074 | 2.101 | 2.013 |
| 2.129 | |||
| 480ng/L | 1.219 | 1.232 | 1.144 |
| 1.245 | |||
| 240ng/L | 0.546 | 0.563 | 0.475 |
| 0.58 | |||
| 120ng/L | 0.307 | 0.295 | 0.207 |
| 0.283 | |||
| 60ng/L | 0.176 | 0.165 | 0.077 |
| 0.153 | |||
| 0ng/L | 0.085 | 0.088 | 0 |
| 0.09 |
Precision
We measured random samples of E4823Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 749.6 | 27 | 3.6 |
| Intra-assay | 2 | 18 | 280.3 | 8.6 | 3.1 |
| Intra-assay | 3 | 18 | 366.1 | 17.9 | 4.9 |
Protocols
Customer reviews and Q&As
Powered by Bioz