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Home > Product > ELISA Kit > Rat Monocyte Chemotactic Protein 2, MCP-2 ELISA KI

Rat Monocyte Chemotactic Protein 2, MCP-2 ELISA KI

Rat Monocyte Chemotactic Protein 2, MCP-2 ELISA KI

Product Summary

Size: 48T,96T

Sensitivity: 1.01ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 1.01ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Monocyte Chemotactic Protein 2

Code

E0194Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.01ng/L

Detection range

2-600ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (By similarity). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By similarity).

UniProt accession

MASS(Da)

16,460

GeneID

24770

Synonyms

C-C motif chemokine 2;CCL 2;CCL2;Immediate-early serum-responsive protein JE;MCP-1;Monocyte chemoattractant protein 1;Monocyte chemotactic protein 1;Small-inducible cytokine A2

Gene names

CCL2

Research area

Immunology

Target protein

CCL2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0194Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400ng/L 2.128 2.106 2.052
2.085
200ng/L 1.4 1.465 1.41
1.53
100ng/L 0.814 0.797 0.742
0.78
50ng/L 0.491 0.486 0.431
0.481
25ng/L 0.272 0.263 0.208
0.254
0ng/L 0.069 0.055 0
0.041

Precision

We measured random samples of E0194Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 103.5 6.12 5.9
Intra-Assay 2 18 206.5 7.9 3.8
Intra-Assay 3 18 48.4 2.82 5.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E0194Ra

    $320.00

  • 96T

    E0194Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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