Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Rat Prostatic Acid Phosphatase, PAP ELISA Kit

Rat Prostatic Acid Phosphatase, PAP ELISA Kit

Rat Prostatic Acid Phosphatase, PAP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Prostatic Acid Phosphatase

Code

E0507Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

[Isoform 1]: A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. [Isoform 2]: In addition to its tyrosine phosphatase activity, also has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP. This extracellular adenosine leads to a decrease in chronic pain by activating A1R in nociceptive neurons.

UniProt accession

MASS(Da)

43,739

GeneID

56780

Synonyms

5'-NT;5'-nucleotidase;Acid phosphatase 3;ACP 3;ACP3;Ecto-5'-nucleotidase;Fluoride-resistant acid phosphatase;FRAP;Prostatic acid phosphatase;Protein tyrosine phosphatase ACP3;Thiamine monophosphatase;TMPase

Gene names

ACP3

Research area

Others

Target protein

ACP3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0507Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 2.027 1.966 1.911
1.905
6ng/mL 1.01 0.998 0.943
0.986
3ng/mL 0.4 0.397 0.342
0.394
1.5ng/mL 0.169 0.218 0.163
0.267
0.75ng/mL 0.115 0.129 0.074
0.144
0ng/mL 0.057 0.055 0
0.054

Precision

We measured random samples of E0507Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5 0.19 3.9
Intra-Assay 2 18 10.7 0.31 2.9
Intra-Assay 3 18 2.9 0.11 3.6

Protocols

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E0507Ra

    $320.00

  • 96T

    E0507Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up