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Home > Product > ELISA Kit > Rat Heat Shock Protein 60, HSP60 ELISA Kit

Rat Heat Shock Protein 60, HSP60 ELISA Kit

Rat Heat Shock Protein 60, HSP60 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Heat Shock Protein 60

Code

E0521Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.

UniProt accession

MASS(Da)

60,955

GeneID

63868

Synonyms

60 kDa chaperonin;60 kDa heat shock protein, mitochondrial;Chaperonin 60;CPN60;Heat shock protein 60;Hsp60;HSP-60;HSP-65;HSPD 1;HSPD1;Mitochondrial matrix protein P1

Gene names

HSPD1

Research area

Others

Target protein

HSPD1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0521Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 2.17 2.26 2.216
2.351
6ng/mL 1.593 1.605 1.56
1.617
3ng/mL 1.171 1.122 1.078
1.074
1.5ng/mL 0.737 0.695 0.65
0.653
0.75ng/mL 0.465 0.434 0.39
0.404
0ng/mL 0.043 0.045 0
0.046

Precision

We measured random samples of E0521Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.7 0.31 5.3
Intra-Assay 2 18 0.9 0.06 7.3
Intra-Assay 3 18 1.9 0.11 5.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E0521Ra

    $320.00

  • 96T

    E0521Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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