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Home > Product > ELISA Kit > Rat Hormone Sensitive Lipase, HSL ELISA Kit

Rat Hormone Sensitive Lipase, HSL ELISA Kit

Rat Hormone Sensitive Lipase, HSL ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.15ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.15ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Hormone Sensitive Lipase

Code

E0922Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.15ng/ml

Detection range

0.3-90ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters (PubMed: 6643478, PubMed: 9162045, PubMed: 10694408). Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of DAGs (By similarity). Preferentially hydrolyzes fatty acids at the sn-1 and sn-2 positions of TAGs (PubMed: 6643478). Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (By similarity). In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production (PubMed: 1770312).

UniProt accession

MASS(Da)

116,812

GeneID

25330

Synonyms

Hormone-sensitive lipase;HSL;LIPE;Monoacylglycerol lipase LIPE;REH;Retinyl ester hydrolase

Gene names

LIPE

Research area

Others

Target protein

LIPE

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0922Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/ml 2.299 2.195 2.156
2.091
24ng/ml 1.716 1.782 1.743
1.848
12ng/ml 1.286 1.282 1.243
1.278
6ng/ml 0.721 0.684 0.645
0.647
3ng/ml 0.415 0.413 0.374
0.41
0ng/ml 0.034 0.039 0
0.044

Precision

We measured random samples of E0922Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 17.9 0.89 5
Intra-Assay 2 18 7.9 0.5 6.3
Intra-Assay 3 18 5.6 0.27 4.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E0922Ra

    $320.00

  • 96T

    E0922Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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