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Home > Product > ELISA Kit > Rat Fructose-1, 6-bisphosphatase 1, FBP1 ELISA Kit

Rat Fructose-1, 6-bisphosphatase 1, FBP1 ELISA Kit

Rat Fructose-1, 6-bisphosphatase 1, FBP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.14ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 9.14ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Fructose-1,6-Bisphosphatase 1

Code

E0977Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.14ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.

UniProt accession

MASS(Da)

39,609

GeneID

24362

Synonyms

D-fructose-1,6-bisphosphate 1-phosphohydrolase 1;FBP 1;FBP1;FBPase 1;Fructose-1,6-bisphosphatase 1;Liver FBPase

Gene names

FBP1

Research area

Others

Target protein

FBP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0977Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.452 2.3 2.233
2.148
1200ng/L 1.361 1.354 1.287
1.347
600ng/L 0.7 0.738 0.67
0.775
300ng/L 0.444 0.442 0.375
0.441
150ng/L 0.33 0.301 0.233
0.271
0ng/L 0.06 0.067 0
0.074

Precision

We measured random samples of E0977Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1650.1 66.29 4
Intra-Assay 2 18 1012.1 54.18 5.4
Intra-Assay 3 18 585.7 42.06 7.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E0977Ra

    $320.00

  • 96T

    E0977Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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