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Home > Product > ELISA Kit > Rat Nuclear Factor Erythroid 2-related Factor 2, NRF2 ELISA Kit

Rat Nuclear Factor Erythroid 2-related Factor 2, NRF2 ELISA Kit

Rat Nuclear Factor Erythroid 2-related Factor 2, NRF2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.07ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.07ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Nuclear Factor Erythroid 2-Related Factor 2

Code

E1083Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.07ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Transcription factor that plays a key role in the response to oxidative stress: binds to antioxidant response (ARE) elements present in the promoter region of many cytoprotective genes, such as phase 2 detoxifying enzymes, and promotes their expression, thereby neutralizing reactive electrophiles (PubMed: 16314513). In normal conditions, ubiquitinated and degraded in the cytoplasm by the BCR(KEAP1) complex. In response to oxidative stress, electrophile metabolites inhibit activity of the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2, heterodimerization with one of the small Maf proteins and binding to ARE elements of cytoprotective target genes (By similarity). The NFE2L2/NRF2 pathway is also activated in response to selective autophagy: autophagy promotes interaction between KEAP1 and SQSTM1/p62 and subsequent inactivation of the BCR(KEAP1) complex, leading to NFE2L2/NRF2 nuclear accumulation and expression of cytoprotective genes (By similarity). May also be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region (By similarity).

UniProt accession

MASS(Da)

67,703

GeneID

83619

Synonyms

NFE2L2;NFE2-related factor 2;NF-E2-related factor 2;Nuclear factor erythroid 2-related factor 2;Nuclear factor, erythroid derived 2, like 2

Gene names

NFE2L2

Research area

Others

Target protein

NFE2L2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1083Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.12 2.254 2.21
2.387
16ng/ml 1.609 1.651 1.608
1.693
8ng/ml 1.031 1.012 0.969
0.994
4ng/ml 0.717 0.703 0.659
0.689
2ng/ml 0.425 0.451 0.407
0.476
0ng/ml 0.027 0.043 0
0.06

Precision

We measured random samples of E1083Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.6 0.23 6.4
Intra-Assay 2 18 5.3 0.3 5.7
Intra-Assay 3 18 12 0.5 4.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E1083Ra

    $320.00

  • 96T

    E1083Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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