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Home > Product > ELISA Kit > Rat Atp-binding Cassette Sub-family G Member 5, ABCG5 ELISA Kit

Rat Atp-binding Cassette Sub-family G Member 5, ABCG5 ELISA Kit

Rat Atp-binding Cassette Sub-family G Member 5, ABCG5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.21ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 7.21ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Atp-Binding Cassette Sub-Family G Member 5

Code

E1246Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.21ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of dietary plant sterols and cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG8 has ATPase activity.

UniProt accession

MASS(Da)

73,372

GeneID

114628

Synonyms

ABCG 5;ABCG5;ATP-binding cassette sub-family G member 5;Sterolin-1

Gene names

ABCG5

Research area

Others

Target protein

ABCG5

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1246Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.439 2.444 2.382
2.449
800ng/L 1.223 1.216 1.153
1.208
400ng/L 0.859 0.87 0.807
0.88
200ng/L 0.548 0.498 0.436
0.449
100ng/L 0.297 0.272 0.21
0.248
0ng/L 0.05 0.062 0
0.074

Precision

We measured random samples of E1246Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 816.3 28.33 3.5
Intra-Assay 2 18 540.7 29.26 5.4
Intra-Assay 3 18 234.3 16.58 7.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E1246Ra

    $320.00

  • 96T

    E1246Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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