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Home > Product > ELISA Kit > Human Suppressors of Cytokine Signaling 3, SOCS-3 ELISA Kit

Human Suppressors of Cytokine Signaling 3, SOCS-3 ELISA Kit

Human Suppressors of Cytokine Signaling 3, SOCS-3 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 9.02ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 9.02ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Suppressors of Cytokine Signaling 3

Code

E0657Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

9.02ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including IL6ST/gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity and regulates IL6 signaling. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed: 15601820).

UniProt accession

MASS(Da)

24,770

GeneID

9021

Synonyms

CIS-3;Cytokine-inducible SH2 protein 3;SOCS 3;SOCS3;SOCS-3;SSI-3;STAT-induced STAT inhibitor 3;Suppressor of cytokine signaling 3

Gene names

SOCS3

Research area

Signal Transduction

Target protein

SOCS3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0657Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.204 2.151 2.087
2.098
1600ng/L 1.426 1.477 1.413
1.528
800ng/L 0.874 0.824 0.76
0.775
400ng/L 0.402 0.466 0.402
0.53
200ng/L 0.243 0.25 0.186
0.257
0ng/L 0.06 0.064 0
0.068

Precision

We measured random samples of E0657Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 646.2 32.35 5
Intra-Assay 2 18 1742.1 67.79 3.9
Intra-Assay 3 18 1254.9 68.08 5.4

Protocols

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Associated products

  • Size

    Code

    Price

  • 96T

    E0657Hu

    $458.00

  • 48T

    E0657Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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