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Home > Product > ELISA Kit > Human Caspase-4, CASP4 ELISA Kit

Human Caspase-4, CASP4 ELISA Kit

Human Caspase-4, CASP4 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.022ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.022ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Caspase-4

Code

E0739Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.022ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Inflammatory caspase that acts as an essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS (PubMed: 23516580, PubMed: 24879791, PubMed: 25119034, PubMed: 22246630, PubMed: 26174085, PubMed: 26173988, PubMed: 26508369, PubMed: 25964352). Thiol protease that cleaves a tetrapeptide after an Asp residue at position P1 (PubMed: 7797510, PubMed: 23516580). Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, followed by IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators (PubMed: 26375003, PubMed: 32109412). Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection: in later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which catalyzes cleavage of GSDMD, resulting in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion (PubMed: 25121752, PubMed: 26375003, PubMed: 25964352, PubMed: 32109412). Cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed: 32109412). Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation (PubMed: 25121752, PubMed: 26375003, PubMed: 25964352). Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity (PubMed: 26508369). Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP Peptides found Alzheimer's patient brains (PubMed: 15123740, PubMed: 22246630, PubMed: 23661706). Activated by direct binding to LPS without the need of an upstream sensor (PubMed: 25119034). Does not directly process IL1B (PubMed: 7743998, PubMed: 7797510, PubMed: 7797592). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed: 28314590).

UniProt accession

MASS(Da)

43,262

GeneID

837

Synonyms

CASP4;CASP-4;Caspase-4;Caspase-4 subunit p10;Caspase-4 subunit p20;ICE and Ced-3 homolog 2;ICE(rel-II;ICH-2;Mih1;Protease TX

Gene names

CASP4

Research area

Others

Target protein

CASP4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0739Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 1.707 1.8 1.744
1.892
6ng/mL 1.122 1.011 0.955
0.901
3ng/mL 0.478 0.536 0.48
0.595
1.5ng/mL 0.21 0.192 0.136
0.174
0.75ng/mL 0.087 0.085 0.029
0.083
0ng/mL 0.056 0.056 0
0.056

Precision

We measured random samples of E0739Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.7 0.27 4.7
Intra-Assay 2 18 12.8 0.43 3.4
Intra-Assay 3 18 3.5 0.14 4.1

Protocols

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Associated products

  • Size

    Code

    Price

  • 96T

    E0739Hu

    $458.00

  • 48T

    E0739Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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