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Home > Product > ELISA Kit > Human Prothrombin, PT ELISA Kit

Human Prothrombin, PT ELISA Kit

Human Prothrombin, PT ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.49U/L

Detection range: 5-2000U/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.49U/L

Detection range: 5-2000U/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Prothrombin

Code

E1146Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.49U/L

Detection range

5-2000U/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.

UniProt accession

MASS(Da)

70,037

GeneID

2147

Synonyms

Activation Peptide fragment 1;Activation Peptide fragment 2;Coagulation factor II;F2;Prothrombin;Thrombin heavy chain;Thrombin light chain

Gene names

F2

Research area

Cardiovascular

Target protein

F2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1146Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200U/L 2.376 2.252 2.202
2.129
600U/L 1.635 1.622 1.571
1.609
300U/L 1.041 1.099 1.048
1.156
150U/L 0.716 0.734 0.684
0.753
75U/L 0.469 0.474 0.423
0.479
0U/L 0.038 0.051 0
0.063

Precision

We measured random samples of E1146Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 457.7 20.67 4.5
Intra-Assay 2 18 244.3 15.78 6.5
Intra-Assay 3 18 145.1 10.74 7.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E1146Hu

    $320.00

  • 96T

    E1146Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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