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Home > Product > ELISA Kit > Human Ubiquitin Carboxyl Terminalhydrolase L1, UCHL1 ELISA Kit

Human Ubiquitin Carboxyl Terminalhydrolase L1, UCHL1 ELISA Kit

Human Ubiquitin Carboxyl Terminalhydrolase L1, UCHL1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.05ng/ml

Detection range: 0.1-38ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.05ng/ml

Detection range: 0.1-38ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ubiquitin Carboxyl Terminal Hydrolase L1

Code

E2328Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.05ng/ml

Detection range

0.1-38ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2 (PubMed: 15694335). Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA (PubMed: 16531995). Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (PubMed: 18082604, PubMed: 26388029).

UniProt accession

MASS(Da)

88,207

GeneID

7398

Synonyms

Deubiquitinating enzyme 1;hUBP;Ubiquitin carboxyl-terminal hydrolase 1;Ubiquitin thioesterase 1;Ubiquitin-specific-processing protease 1;USP 1;USP1

Gene names

USP1

Research area

Others

Target protein

USP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2328Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
20ng/ml 2.245 2.235 2.205
2.224
10ng/ml 1.715 1.641 1.611
1.566
5ng/ml 0.992 1.038 1.008
1.083
2.5ng/ml 0.684 0.677 0.648
0.67
1.25ng/ml 0.417 0.448 0.419
0.479
0ng/ml 0.028 0.029 0
0.03

Precision

We measured random samples of E2328Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2 0.16 7.8
Intra-Assay 2 18 12 0.53 4.4
Intra-Assay 3 18 3.8 0.28 7.4

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2328Hu

    $320.00

  • 96T

    E2328Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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