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Home > Product > ELISA Kit > Human Ficolin 1, FCN1 ELISA Kit

Human Ficolin 1, FCN1 ELISA Kit

Human Ficolin 1, FCN1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.92ng/ml

Detection range: 2-600ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.92ng/ml

Detection range: 2-600ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ficolin 1

Code

E3143Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.92ng/ml

Detection range

2-600ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Extracellular lectin functioning as a pattern-recognition receptor in innate immunity. Binds the sugar moieties of pathogen-associated molecular patterns (PAMPs) displayed on microbes and activates the lectin pathway of the complement system. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8 (PubMed: 21037097). Binds preferentially to 9-O-acetylated 2-6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage.

UniProt accession

MASS(Da)

35,078

GeneID

2219

Synonyms

Collagen/fibrinogen domain-containing protein 1;FCN 1;FCN1;Ficolin-1;Ficolin-A;Ficolin-alpha;M-ficolin

Gene names

FCN1

Research area

Others

Target protein

FCN1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3143Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400ng/ml 2.05 2.076 2.037
2.103
200ng/ml 1.807 1.75 1.711
1.694
100ng/ml 1.226 1.247 1.207
1.267
50ng/ml 0.625 0.635 0.595
0.644
25ng/ml 0.395 0.397 0.358
0.399
0ng/ml 0.027 0.04 0
0.052

Precision

We measured random samples of E3143Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 119.4 6.01 5
Intra-Assay 2 18 92.3 5.91 6.4
Intra-Assay 3 18 45.7 2.42 5.3

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E3143Hu

    $320.00

  • 96T

    E3143Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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