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Home > Product > ELISA Kit > Human Ninein/Gsk3B Interacting Protein, NIN ELISA Kit

Human Ninein/Gsk3B Interacting Protein, NIN ELISA Kit

Human Ninein/Gsk3B Interacting Protein, NIN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.05ng/ml

Detection range: 0.1-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.05ng/ml

Detection range: 0.1-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ninein/Gsk3B Interacting Protein

Code

E3201Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.05ng/ml

Detection range

0.1-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

A-kinase anchoring protein for GSK3B and PKA that regulates or facilitates their kinase activity towards their targets (PubMed: 27484798, PubMed: 25920809, PubMed: 16981698). The ternary complex enhances Wnt-induced signaling by facilitating the GSK3B- and PKA-induced phosphorylation of beta-catenin leading to beta-catenin degradation and stabilization respectively (PubMed: 27484798, PubMed: 16981698). Upon cAMP activation, the ternary complex contributes to neuroprotection against oxidative stress-induced apoptosis by facilitating the PKA-induced phosphorylation of DML1 and PKA-induced inactivation of GSK3B (PubMed: 25920809). During neurite outgrowth promotes neuron proliferation; while increases beta-catenin-induced transcriptional activity through GSK3B kinase activity inhibition, reduces N-cadherin level to promote cell cycle progression (PubMed: 19830702).

UniProt accession

MASS(Da)

15,648

GeneID

51527

Synonyms

GSK3beta interaction protein;GSK3B-interacting protein;GSKIP

Gene names

GSKIP

Research area

Cell Biology

Target protein

GSKIP

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3201Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.127 2.255 2.198
2.384
8ng/ml 1.239 1.255 1.197
1.271
4ng/ml 0.798 0.82 0.762
0.842
2ng/ml 0.419 0.417 0.36
0.416
1ng/ml 0.298 0.282 0.224
0.265
0ng/ml 0.05 0.058 0
0.065

Precision

We measured random samples of E3201Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 11.9 0.38 3.2
Intra-Assay 2 18 6.5 0.39 6.1
Intra-Assay 3 18 3.3 0.2 6.1

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E3201Hu

    $320.00

  • 96T

    E3201Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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