Product overview
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Background
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
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Target protein
Components
Components | Quantity |
---|---|
Pre-coated ELISA plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate (25x) | 20ml x1 |
Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0004Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
800ng/L | 2.127 | 2.238 | 2.182 |
2.35 | |||
400ng/L | 1.368 | 1.378 | 1.322 |
1.389 | |||
200ng/L | 0.847 | 0.78 | 0.723 |
0.713 | |||
100ng/L | 0.464 | 0.437 | 0.38 |
0.41 | |||
50ng/L | 0.243 | 0.285 | 0.228 |
0.326 | |||
0ng/L | 0.064 | 0.057 | 0 |
0.049 |
Precision
We measured random samples of E0004Rb within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-Assay | 1 | 18 | 82.78 | 4.9 | 5.92 |
Intra-Assay | 2 | 18 | 585.71 | 20.73 | 3.54 |
Intra-Assay | 3 | 18 | 239.41 | 12.37 | 5.17 |
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