Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Sheep Cyclooxygenase-1, COX-1 ELISA Kit

Sheep Cyclooxygenase-1, COX-1 ELISA Kit

Sheep Cyclooxygenase-1, COX-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.12ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Sheep

Product Summary

Size: 48T,96T

Sensitivity: 0.12ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Sheep

Product overview

Full product name

cyclooxygenase-1

Code

E0221Sh

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.12ng/ml

Detection range

0.2-70ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Sheep

Storage

2-8ºC

Assay time

1h 30m

Background

Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate, with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates arachidonate (AA, C20:4(n-6)) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide PGH2, the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons (PubMed:7947975). Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells (PubMed:10438452).

UniProt accession

MASS(Da)

68,861

GeneID

443551

Synonyms

COX1;COX-1;Cyclooxygenase-1;PGH Synthase 1;PGHS-1;PHS 1;Prostaglandin G/H Synthase 1;Prostaglandin H2 Synthase 1;Prostaglandin-endoperoxide Synthase 1;

Gene names

PTGS1

Research area

Immunology

Target protein

COX-1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0221Sh are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 2.311 2.269 2.249
2.227
20ng/ml 1.31 1.282 1.262
1.254
10ng/ml 0.571 0.66 0.64
0.748
5ng/ml 0.434 0.378 0.358
0.322
2.5ng/ml 0.132 0.13 0.11
0.128
0ng/ml 0.016 0.02 0
0.024

Precision

We measured random samples of E0221Sh within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 19.27 0.69 3.56
Intra-Assay 2 18 13.63 0.58 4.24
Intra-Assay 3 18 4.92 0.28 5.63

Protocols

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E0221Sh

    $320.00

  • 96T

    E0221Sh

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up