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Home > Product > Antibody > BCL2 Monoclonal Antibody

BCL2 Monoclonal Antibody

BCL2 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,FCM

Product overview

Code

BT-MCA2509

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human BCL2 (AA: 1-239) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Uniprot accession

Molecular weight

26.3kDa

Gene ID

596

Synonyms

Bcl-2;PPP1R50

Research area

Cell Biology

Target protein

BCL2

Recommended dilution

Application Dilution
WB 1:500 - 1:2000
FCM 1:200 - 1:400
ELISA 1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using BCL2 mAb against human BCL2 (AA: 1-239) recombinant protein. (Expected MW is 29.2 kDa)
Western blot analysis using BCL2 mAb against HEK293-6e (1) and BCL2 (AA: 1-239)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western blot analysis using BCL2 mouse mAb against Raji (1), MCF-7 (2), Jurkat (3), A549 (4), HEK293 (5), T47D (6), Hela (7), and C6 (8) cell lysate.
Flow cytometric analysis of THP-1 cells using BCL2 mouse mAb (green) and negative control (red).

Protocols

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Product size

Price

  • 100μL

    $320.00

  • 50μL

    $220.00

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Lead time: Within one week

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