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Home > Product > ELISA Kit > Sheep Sorbitol Dehydrogenase, SDH ELISA Kit

Sheep Sorbitol Dehydrogenase, SDH ELISA Kit

Sheep Sorbitol Dehydrogenase, SDH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.098ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Sheep

Product Summary

Size: 48T,96T

Sensitivity: 0.098ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Sheep

Product overview

Full product name

Sorbitol Dehydrogenase

Code

E0143Sh

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.098ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Sheep

Storage

2-8ºC

Assay time

1h 30m

Background

Polyol dehydrogenase that catalyzes the reversible NAD+-dependent oxidation of various sugar alcohols. Is mostly active with xylitol, L-iditol and D-sorbitol (D-glucitol) as substrates, leading to the C2-oxidized products D-xylulose, L-sorbose and D-fructose, respectively (PubMed:1459146). Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism (By similarity). May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility (By similarity).

UniProt accession

MASS(Da)

37,831

GeneID

101115280

Synonyms

L-iditol 2-dehydrogenase;Polyol dehydrogenase;SDH;Sorbitol dehydrogenase;sord;Xylitol dehydrogenase

Gene names

SORD

Research area

Signal Transduction; Metabolism

Target protein

SDH

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0143Sh are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
36ng/ml 2.147 2.175 2.131
2.202
18ng/ml 1.949 1.947 1.903
1.945
9ng/ml 1.164 1.176 1.132
1.188
4.5ng/ml 0.662 0.693 0.65
0.725
2.25ng/ml 0.4 0.408 0.364
0.415
0ng/ml 0.035 0.044 0
0.052

Precision

We measured random samples of E0143Sh within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 8.19 0.47 5.76
Intra-Assay 2 18 15.37 0.8 5.2
Intra-Assay 3 18 3.95 0.17 4.25

Protocols

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  • Size

    Code

    Price

  • 48T

    E0143Sh

    $320.00

  • 96T

    E0143Sh

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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