Product name
Recombinant Mouse MIP-1 Gamma/CCL9/CCL10 protein
Code
CD00296
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml.
Endotoxin
Less than 1 EU/μg of rMuMIP-1γ/CCL9/CCL10 as determined by LAL method.
Synonyms
MIP-1γ/CCL9/CCL10, MRP; Scy; Scya; CCF18; MRP-2; Scya9; Scya10,Mouse
Sequence
QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q
Uniprot Accession
Molecular Weight
Approximately 11.6 kDa, a single non-glycosylated polypeptide chain containing 101 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
Mouse CCL9/10 is belonging to the CC chemokine family. It is encoded by the gene CCL9. MRP-2 (CCL9/10) was isolated from a Mouse macrophage cell line, RAW 264.7. MRP-2 shows a 51 % sequence identity at the protein level to MRP-1 (C10) and 46% identity to MIP-1-alpha (CCL3). The functional receptor for CCL9/10 has been identified as CCR1. In contrast, other CCR1 ligands, CCL3/MIP-1alpha and CCL5/RANTES are considered to be primary CCR1 ligands in inflammatory responses. CCL9 can activate osteoclasts suggesting an important role for it in bone resorption. Mouse CCL9/10 is 75 % amino acid identical to rat CCL9/10.
Storage
-20ºC
Research area
Immunology
